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Thermo Scientific™ TMT™ Elution Buffer

Thermo Scientific™ TMT™ Elution Buffer is for use with Thermo Scientific™ Immobilized Anti-TMT™ Resin to enrich samples for peptides that have been chemically labeled with Tandem Mass Tag™ (TMT™) Reagents.

Manufacturer:  Thermo Scientific™ 0090104

Catalog No. PI90104



Description

Description

Immobilized Anti-TMT Resin and TMT Elution Buffer are effective for reducing sample complexity, improving dynamic range and studying cysteine modifications such as S-nitrosylation, oxidation and disulfide bonds of protein samples labeled with iodoTMT Reagents. This approach of selective thiol labeling and affinity enrichment is similar to isotope-coded affinity tags (ICAT™ Method) but allows for higher multiplex quantitation.

The Immobilized Anti-TMT Resin uses the highly specific Anti-TMT Antibody to bind peptides labeled with TMT Reagents. The antibody is specific for the mass reporter region of the TMT Reagents which allows for enrichment of TMT-labeled peptides regardless of peptide labeling chemistry (e.g., amine-reactive, sulfhydryl-reactive). The TMT Elution Buffer is a volatile neutral buffer which competitively elutes captured TMT-labeled peptides. When used in combination with the Immobilized Anti-TMT Resin, the TMT Elution Buffer provides higher specific enrichment of TMT-labeled peptides compared to acidic or basic elution buffers.

Specifications

Specifications

Store at 4°C.
Mass Spectrometry
Cell Lysate
Mass Spectrometer
Tandem Mass Tag™ (TMT) Labels
Peptide
Protein Labeling
TMT™
SDS
Documents
Product Certifications
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