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Thermo Scientific™ Lab Vision™ Tid-1 Ab-2, Mouse Monoclonal Antibody


Provide accurate, reproducible results with the Thermo Scientific™ Tid-1 Ab-2, Mouse Monoclonal Antibody.

Manufacturer: thermo scientific™ lab vision™  MS1564P1ABX

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Catalog No. MS1564P1ABX

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Description & Specifications

Specifications

Immunogen Recombinant hTid-1 protein
Regulatory Status RUO
Cross Reactivity Human
Cross Reactivity Mouse
Cross Reactivity Rat
Antigen Tid-1 Ab-2
Isotype IgG2a kappa
Host Species Mouse
Applications Immunoprecipitation
Applications Western Blotting
Quantity 100µL
Clone RS13
Format Concentrated
Purification Method HeLa and Jurkat cells
Research Discipline Apoptosis
Type Primary
Classification Monoclonal
Concentration 1mg/mL
Conjugate Unlabeled

Provide accurate, reproducible results with the Thermo Scientific™ Tid-1 Ab-2, Mouse Monoclonal Antibody.

TID1 is a human homolog of the Drosophila tumor suppressor lethal (2) tumerous imaginal discs, l(2)tid and encodes two mitochondrial matrix localized splice variants of human Tid-1 designated hTid-1S and hTid-1L. These proteins are the conserved members of the DnaJ family of proteins which act as cochaperons for mitochondrial Hsp70. They contain a conserved tetrahedrical J domain which binds to Hsp70 chaperones and activates their ATPase activity. Expression of hTid-1L increases apoptosis induced by DNA damaging agents as mitomycin-C and TNF-α. A J-domain mutant of hTid-1L can dominantly suppress apoptosis and in sharp contrast the J-domain mutant of hTid-1S increases apoptosis. Expression of hTid-1S and hTid-1L affects cytochrome c release from the mitochondria and caspase 3 activation, while activation of caspase 8 is unaffected. It is strongly suggested that these two splice variants exert their anti- and pro- apoptotic effects through discrete substrates and actiuvities. Hence the relative abundance of these proteins or their substrates may allow the mitochondria to dampen or enhance the apoptotic signals.

Host Species: Mouse

Clone: RS13

Isotype: IgG1/κ

Species Reactivity: Human, Mouse and Rat. Others not known.

Immunogen: Recombinant hTid-1 protein

Molecular Weight: 40 and 43kDa

Positive Control: HeLa and Jurkat cells

Cellular Localization: Mitochondria

Recommended for:

  • Western Blotting
  • Immunoprecipitation

Note:

USA: RUO; Int'l: RUO