Thermo Scientific™ Lab Vision™ Tid-1 Ab-2, Mouse Monoclonal Antibody
Provide accurate, reproducible results with the Thermo Scientific™ Tid-1 Ab-2, Mouse Monoclonal Antibody.
Manufacturer: Thermo Scientific™ Lab Vision™ MS1564PABX
Provide accurate, reproducible results with the Thermo Scientific™ Tid-1 Ab-2, Mouse Monoclonal Antibody.TID1 is a human homolog of the Drosophila tumor suppressor lethal (2) tumerous imaginal discs, l(2)tid and encodes two mitochondrial matrix localized splice variants of human Tid-1 designated hTid-1S and hTid-1L. These proteins are the conserved members of the DnaJ family of proteins which act as cochaperons for mitochondrial Hsp70. They contain a conserved tetrahedrical J domain which binds to Hsp70 chaperones and activates their ATPase activity. Expression of hTid-1L increases apoptosis induced by DNA damaging agents as mitomycin-C and TNF-α. A J-domain mutant of hTid-1L can dominantly suppress apoptosis and in sharp contrast the J-domain mutant of hTid-1S increases apoptosis. Expression of hTid-1S and hTid-1L affects cytochrome c release from the mitochondria and caspase 3 activation, while activation of caspase 8 is unaffected. It is strongly suggested that these two splice variants exert their anti- and pro- apoptotic effects through discrete substrates and actiuvities. Hence the relative abundance of these proteins or their substrates may allow the mitochondria to dampen or enhance the apoptotic signals.
Host Species: Mouse
Species Reactivity: Human, Mouse and Rat. Others not known.
Immunogen: Recombinant hTid-1 protein
Molecular Weight: 40 and 43kDa
Positive Control: HeLa and Jurkat cells
Cellular Localization: Mitochondria
- Western Blotting
USA: RUO; Int'l: RUO
|Recombinant hTid-1 protein|
|HeLa and Jurkat cells|