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RuvB Recombinant Protein, Invitrogen™


RuvB Recombinant Protein, RP-4908, from Invitrogen™

Manufacturer: pierce biotechnology inc  RP4908

Catalog No. ARC4908

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Description & Specifications

Specifications

Conjugate Unlabeled
For Use With (Application) Control, Western Blot
Format Purified
Formulation 20μg purified protein (2mg/mL) in TBS with 50% glycerol, 5mM beta mercaptoethanol, 2mM EDTA and no preservative; pH 7.5
Molecular Weight ∽37
Name RuvB Recombinant Protein
pH Range 7.5
Purification Method Chromatography
Quantity 20μg
Recombinant Yes
Source E. coli
Storage Requirements store at -20°C, Avoid Freeze/Thaw Cycles

RuvB Recombinant Protein, RP-4908, from Invitrogen.

E. coli RuvB protein forms a complex with RuvA protein at the late stage of homologous recombination and recombination repair and binds specifically to the Holliday structure which is the intermediate of recombination, allowing the migration of Holliday junction using ATP hydrolysis energy and expands the heteroduplex region. RuvB forms a hexamer ring structure and surrounds the double chain DNA and covers RuvA tetramer bound to the Holliday junction from both sides. RuvB is a DNA motor protein which possesses the ATPase activity, activated by DNA and RuvA protein. Its molecular weight is 37 kD and forms a dimer in solution in the physiological condition.
Host Species: E. Coli
Formulation: 20μg purified protein (2mg/mL) in TBS with 50% glycerol, 5mM beta mercaptoethanol, 2mM EDTA and no preservative; pH 7.5
RP-4908 can be used as a positive control in Western blotting, as well as in studies on homologous recombination mechanism. RP-4908 calculated molecular weight is ∽37 kDa. Purity is 90%.