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RuvA Recombinant Protein, Invitrogen™


RuvA Recombinant Protein, RP-4906, from Invitrogen™

Manufacturer: pierce biotechnology inc  RP4906

Catalog No. ARC4906

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Description & Specifications

Specifications

Concentration 2.7mg/mL
Conjugate Unlabeled
For Use With (Application) Control, Western Blot
Format Purified
Formulation 20μg purified protein (2.7mg/mL) in TBS with 50% glycerol, 5mM beta mercaptoethanol, 2mM EDTA and no preservative; pH 7.5
Molecular Weight ∽22
Name RuvA Recombinant Protein
pH Range 7.5
Purification Method Chromatography
Quantity 20μg
Recombinant Yes
Source E. coli
Storage Requirements store at -20°C, Avoid Freeze/Thaw Cycles

RuvA Recombinant Protein, RP-4906, from Invitrogen.

E. coli RuvA protein binds specifically to the Holliday structure which is the intermediate of recombination at the late stage of homologous recombination and recombination repair and forms a complex with RuvB motor protein allowing the migration of Holliday junction using ATP hydrolysis energy and expands the heteroduplex region. In solution, it forms a tetramer and binds to the cross-like DNA of the Holliday junction from below and above holding it in between.
Host Species: E. Coli
Formulation: 20μg purified protein (2.7mg/mL) in TBS with 50% glycerol, 5mM beta mercaptoethanol, 2mM EDTA and no preservative; pH 7.5
RP-4906 can be used as a positive control in Western blotting, as well as in studies on homologous recombination mechanism. RP-4906 calculated molecular weight is ∽22 kDa. Purity is 90%.