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RecA Recombinant Protein, Invitrogen™


RecA Recombinant Protein, RP-4900, from Invitrogen™

Manufacturer: pierce biotechnology inc  RP4900

Catalog No. ARC4900

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Description & Specifications

Specifications

Concentration 0.9mg/mL
Conjugate Unlabeled
Cross Reactivity Human
For Use With (Application) Control, Western Blot
Format Purified
Formulation 100μg purified protein (1.6mg/mL) in TBS with 0.15M KCl, 50% glycerol, 1mM DTT, 1mM EDTA and no preservative; pH 8.0
Molecular Weight ∽38
Name Human Rad51 Recombinant Protein
pH Range 8
Quantity 100μg
Recombinant Yes
Source E. coli
Storage Requirements store at -20°C, Avoid Freeze/Thaw Cycles
Gene Alias RAD51A

RecA Recombinant Protein, RP-4900, from Invitrogen.

E. coli RecA protein is a very important enzyme for homologous recombination and recombinational repair. Expression is induced by SOS response caused by DNA damage. The RecA protein has multiple functions, such as single stranded DNA dependent ATPase activity, DNA annealing activity, formation of D-loops and Holliday structures during homologous recombination, and co-protease activities that promote self-cleavage of the LexA repressor, lambda phage repressor and UmuD protein. RecA protein binds to single and double stranded DNA for nucleofilament formation. It carries out a central role in homologous recombination. Its is homologous to the eukaryotic Rad51 protein and Dmcl protein.
Host Species: E. Coli
Formulation: 100μg purified protein (1.6mg/mL) in TBS with 0.15M KCl, 50% glycerol, 1mM DTT, 1mM EDTA and no preservative; pH 8.0
RP-4900 can be used as a positive control for Western blots with polyclonal RecA (PA1-4924). This product can also be used for studies of the homologous recombination mechanism and SOS response, to facilitate hybridization during library screening using a DNA probe, and to facilitate observation of nucleofilament formation of DNA by electron microscopy. The Human gene Rad51 is homologous to the highly conserved bacterial gene RecA. RP-4900 calculated molecular weight is ∽38 kDa.