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Thermo Scientific™ Pierce™ Quantitative Fluorometric Peptide Assay Kit

Measure total peptide concentration by a mix-and-read fluorescent microplate method that provides improved sensitivity, reproducibility and ease using the Thermo Scientific™ Pierce™ Quantitative Fluorescent Peptide Assay.

Manufacturer:  Thermo Scientific™ 23295

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Catalog No. PI23295



Description

Description

The Thermo Scientific Pierce Quantitative Fluorescent Peptide Assay is a sensitive, mix-and-read fluorescent microplate assay for the quantitative measurement of peptides and peptide mixtures.

  • Sensitive - accurately detect as little as 5.0μg/mL of single peptides or peptide mixtures
  • Robust - assay performance rigorously tested using both peptides and peptide digest mixtures
  • Robust peptide digest standard - kit includes a validated peptide digest standard for improved reproducibility of quantitation
  • Compatible - works with many reagents, including those used in mass spectrometry sample preparation
  • Convenient - easy mix-and-read format and stable fluorescent signal that may be read in as little as 5 minutes up to several hours
The Pierce Quantitative Fluorescent Peptide Assay reagents include peptide assay buffer, fluorescent peptide labeling reagent, and a peptide digest assay standard for the quantitative measurement of peptide concentrations. In this assay, peptides are specifically labeled at the amino-terminus using an amine-reactive fluorescent reagent, and the fluorescently labeled peptides are detected using ex390/em475.
Because of the labeling mechanism of the fluorescent assay reagent, this assay is suitable for the quantitative measurement of synthetic peptides as well as peptide digest mixtures. A high quality peptide digest reference standard is included for use in generating linear standard curves and as a calibration control for improved accuracy and reproducibility. This sensitive assay requires only 10μL of sample, produces a linear response with increasing peptide concentrations (5 to 1000μg/mL), and results in a stable final fluorescence that can be detected in as little as five minutes.
Applications:
  • Recommended for use with peptide mixtures and synthetic peptides with an unblocked amino-terminus (amino-terminus-labeled peptides, such as TMT-labeled peptides, are not compatible with this assay)
  • Normalizing sample concentrations for quantitative downstream applications
  • Normalizing sample loading volumes for LC, MS, and LC/MS applications
  • Measuring recovery after peptide clean-up procedures

Specifications

Specifications

Fluorescent, colorimetric
Microplate reader
Protein assays and analysis, protein mass spectrometry, protein biology, protein quantitation, sample prep for mass spectrometry
1.5mL
Preparing 10 sets of nine-point assay standard curves for microplate assays run in triplicate
Peptide Digest Assay Standard, 1.5mL, Fluorescent, Colorimetric Detection Method, 2 to 8°C Storage, For Use With Equipment Microplate Reader, is a Validated Protein Digest Reference Sample Optimized For Quantitative Peptide Assays
1mg/mL solution of tryptic digest of a protein standard, in 50mM ammonium bicarbonate and 0.05% sodium azide
Peptide Digest Assay Standard (1 mg/mL) for Pierce™ Quantitative Peptide Assays
Store in refrigerator (2 to 8°C).
2 to 8°C
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