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Thermo Scientific™ Pierce™ Quantitative Fluorometric Peptide Assay Kit

Measure total peptide concentration by a mix-and-read fluorescent microplate method that provides improved sensitivity, reproducibility and ease using the Thermo Scientific™ Pierce™ Quantitative Fluorescent Peptide Assay.

Manufacturer:  Thermo Scientific™ 23290

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Catalog No. PI23290



Description

Description

The Thermo Scientific Pierce Quantitative Fluorescent Peptide Assay is a sensitive, mix-and-read fluorescent microplate assay for the quantitative measurement of peptides and peptide mixtures.

  • Sensitive - accurately detect as little as 5.0μg/mL of single peptides or peptide mixtures
  • Robust - assay performance rigorously tested using both peptides and peptide digest mixtures
  • Robust peptide digest standard - kit includes a validated peptide digest standard for improved reproducibility of quantitation
  • Compatible - works with many reagents, including those used in mass spectrometry sample preparation
  • Convenient - easy mix-and-read format and stable fluorescent signal that may be read in as little as 5 minutes up to several hours
The Pierce Quantitative Fluorescent Peptide Assay reagents include peptide assay buffer, fluorescent peptide labeling reagent, and a peptide digest assay standard for the quantitative measurement of peptide concentrations. In this assay, peptides are specifically labeled at the amino-terminus using an amine-reactive fluorescent reagent, and the fluorescently labeled peptides are detected using ex390/em475.
Because of the labeling mechanism of the fluorescent assay reagent, this assay is suitable for the quantitative measurement of synthetic peptides as well as peptide digest mixtures. A high quality peptide digest reference standard is included for use in generating linear standard curves and as a calibration control for improved accuracy and reproducibility. This sensitive assay requires only 10μL of sample, produces a linear response with increasing peptide concentrations (5 to 1000μg/mL), and results in a stable final fluorescence that can be detected in as little as five minutes.
Applications:
  • Recommended for use with peptide mixtures and synthetic peptides with an unblocked amino-terminus (amino-terminus-labeled peptides, such as TMT-labeled peptides, are not compatible with this assay)
  • Normalizing sample concentrations for quantitative downstream applications
  • Normalizing sample loading volumes for LC, MS, and LC/MS applications
  • Measuring recovery after peptide clean-up procedures

Specifications

Specifications

Fluorescent
Microplate reader
Recommended for use with peptide mixtures and synthetic peptides with an unblocked amino-terminus (amino-terminus-labeled peptides, such a TMT-labeled peptides, are not compatible with this assay), normalizing sample concentrations for quantitative downst
Fluorometric peptide assay reagent, 4 x 2.5mL, fluorometric peptide assay buffer, 50mL, peptide digest assay standard, 1.5mL
500 assays, 500 Assay(s)
500 microplate assays with 10μL of samples, 500 Assay(s)
Quantitative Fluorometric Peptide Assay, 500 Assay, Fluorescent Detection Method, 2 to 8°C Storage, For Use With Equipment Microplate Reader, Recommended For Use With Peptide Mixtures and Synthetic
Multi-component kit with assay reagents and standard
Fluorometric peptide assay reagent, 4 x 2.5mL, fluorometric peptide assay buffer, 50mL, peptide digest assay standard, 1.5mL
Pierce™ Quantitative Fluorometric Peptide Assay Kit,Quantitative Fluorometric Peptide Assay
Store in refrigerator (2 to 8°C)., 2 to 8°C
2 to 8°C
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