Phalloidin DyLight 554, Invitrogen™
Phalloidin Control, DyLight 554 conjugate, 21834, from Invitrogen™., 21834, from Invitrogen™. Species Reactivity: Many; Applications: ICC, IF, IHC.
Manufacturer: Pierce Biotechnology Inc 21834*
Phalloidin Control, DyLight 554 conjugate, 21834, from Invitrogen., 21834, from Invitrogen. Species Reactivity: Many; Applications: Immunocytochemistry, Immunofluorescence, Immunohistochemistry.Phalloidin is a bicyclic peptide that belongs to a family of toxins isolated from the deadly Amanita phalloides “death cap” mushroom and is commonly used as a counterstain (similar to DAPI or Hoechst) in cell biology and histology imaging applications to selectively label F-actin in fixed cells, permeabilized cells, and cell-free experiments. Labeled phalloidin conjugates have similar affinity for both large and small filaments and bind in a stoichiometric ratio of about one phallotoxin per actin subunit in both muscle and non-muscle cells. Phalloidins reportedly do not bind to monomeric G-actin, unlike some antibodies against actin. The dynamics of the actin polymerization in cells are important for a variety of cellular processes from cell motility to cell shape, from muscular contraction to cytokinesis, and more.
Cross Reactivity: Many Species
Formulation: 300 units purified control antibody (300 units/ml) in methanol and no preservative
Synonyms: DY554-Phalloidin, F-actin toxin, Amanita phalloides
Format: 300 units of lyophilized DyLight 554-Phalloidin (300 units/ml) in methanol. Prepare stock solution by adding 1000 µL of pure methanol to the vial and gently mix. 21834 has been successfully used in immunofluorescence and immunohistochemistry. DyLight 554-Phalloidin has an excitation/emission of 551/572nm and molecular weight of 1313.5 g/mole. DyLight 554-Phalloidin Stock Solution can be prepared in methanol, DMF, or DMSO and unused Stock Solution should be promptly stored at -20°C to minimize evaporation. Working solutions (per 96-well plate) can be prepared by diluting 20 µL of the Stock Solution in 5.98mL of PBS and mixing well. Typical staining procedure adds 50µl of Working Solution (i.e. 1 to 5 unit/ml) to a each well. Incubate cells in the dark for 30 minutes at room temperature (optimal staining times varies from 10 minutes to 3 hours depending on cell type). Aspirate and wash cells three times in PBS after incubation. If desired, probe with specific antibodies or dyes before using DyLight 554-Phalloidin.
|DY554-Phalloidin, F-actin toxin, Amanita phalloides|
|300 units purified control antibody (300 units/ml) in methanol and no preservative|
|Store at -20°C|