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LexA Repressor Recombinant Protein, Invitrogen™


LexA Repressor Recombinant Protein, RP-4905, from Invitrogen™

Manufacturer: pierce biotechnology inc  RP4905

Catalog No. ARC4905

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Description & Specifications

Specifications

Formulation 100μg purified protein (0.8mg/mL) in TBS with 50% glycerol, 5mM beta mercaptoethanol, 2mM EDTA and no preservative; pH 7.5
For Use With (Application) Control, Western Blot
Conjugate Unlabeled
Host Species E. Coli
Format Purified
Storage Requirements store at -20°C, Avoid Freeze/Thaw Cycles
pH Range 7.5
Molecular Weight 23
Name LexA Repressor
Recombinant Yes
Source E. coli
Quantity 100μg

LexA Repressor Recombinant Protein, RP-4905, from Invitrogen.

E. coli LexA protein inhibits the transcription of the genes belonging to the SOS regulon that are related to DNA repair and cell division by recognizing and binding to the SOS-box sequence. LexA‘s self-protease activity is promoted by RecA protein which, responding to DNA damage, is activated by its binding to single-strand DNA accumulated in the cells. It is cleaved into two fragments and loses its function as a repressor. As the result, the expression of genes belonging to the SOS regulon is induced, and mutagenic activity in the cells are enhanced.
Host Species: E. Coli
Formulation: 100μg purified protein (0.8mg/mL) in TBS with 50% glycerol, 5mM beta mercaptoethanol, 2mM EDTA and no preservative; pH 7.5
RP-4905 can be used as a positive control in Western blotting to confirm that the Bait construct is expressed stably in the nucleus, as well as in studies on the mechanism of E. coli SOS response. RP-4905 calculated molecular weight is ∽23 kDa. Purity is 90%.