Please login to your online account to display your discounted pricing

LexA Repressor Recombinant Protein, Invitrogen™


LexA Repressor Recombinant Protein, RP-4904, from Invitrogen™

Manufacturer: pierce biotechnology inc  RP4904

Catalog No. ARC4904

  • / Each
 

Description & Specifications

Specifications

Conjugate Unlabeled
For Use With (Application) Control, Western Blot
Format Purified
Formulation 20μg purified protein (0.8mg/mL) in TBS with 50% glycerol, 5mM beta mercaptoethanol, 2mM EDTA and no preservative; pH 7.5
Molecular Weight 23
Name LexA Repressor
pH Range 7.5
Quantity 20μg
Recombinant Yes
Source E. coli
Storage Requirements store at -20°C, Avoid Freeze/Thaw Cycles

LexA Repressor Recombinant Protein, RP-4904, from Invitrogen.

E. coli LexA protein inhibits the transcription of the genes belonging to the SOS regulon that are related to DNA repair and cell division by recognizing and binding to the SOS-box sequence. LexA‘s self-protease activity is promoted by RecA protein which, responding to DNA damage, is activated by its binding to single-strand DNA accumulated in the cells. It is cleaved into two fragments and loses its function as a repressor. As the result, the expression of genes belonging to the SOS regulon is induced, and mutagenic activity in the cells are enhanced.
Host Species: E. Coli
Formulation: 20μg purified protein (0.8mg/mL) in TBS with 50% glycerol, 5mM beta mercaptoethanol, 2mM EDTA and no preservative; pH 7.5
RP-4904 can be used as a positive control in Western blotting to confirm that the Bait construct is expressed stably in the nucleus, as well as in studies on the mechanism of E. coli SOS response. RP-4904 calculated molecular weight is ∽23 kDa. Purity is 90%.