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anti-Fibroblast Growth Factor basic (human) Recombinant, Thermo Scientific™


Fibroblast Growth Factor basic (Hu) Recombinant protein RP-8627 detects Fibroblast Growth Factor basic (Hu) in Not Applicable samples, and is validated for Control

Manufacturer: pierce biotechnology inc  RP8628

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Catalog No. PIRP8628

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Description & Specifications

Specifications

Accession Number P09038
Concentration 0.1mg/mL
Conjugate Unlabeled
Cross Reactivity Human
For Use With (Application) Control
Format Purified
Formulation 50μg purified protein (0.1mg/mL) in 10mM sodium phosphate with 75mM NaCl and no preservative; pH 8.0
Gene ID (Entrez) 2247
Molecular Weight ∽17.2
Name Human Fibroblast Growth Factor basic Recombinant Protein
pH Range 8
Quantity 50μg
Recombinant Yes
Source E. coli
Storage Requirements store at -20°C, Avoid Freeze/Thaw Cycles
Gene Alias H-FGF-b-154
Gene Symbol FGF2

Fibroblast Growth Factor basic (Hu) Recombinant protein RP-8627 detects Fibroblast Growth Factor basic (Hu) in Not Applicable samples, and is validated for Control

Fibroblast Growth Factors (FGFs) are a 22 member family of proteins known to be involved in angiogenesis, wound healing and embryonic development. As a family, they bind to heparin and signal through four receptor tyrosine kinases called, FGFR1, 2, 3 and 4. Although the mechanism remains unclear, FGF-basic 154, also known as FGF-2, is a critical component in keeping embryonic stem cells undifferentiated in cell culture systems.
Host Species: E. Coli
Formulation: 50μg purified protein (0.1mg/mL) in 10mM sodium phosphate with 75mM NaCl and no preservative; pH 8.0
Recombinant human FGF-b 154 (FGF-2) is a non-glycosylated protein, containing 154 amino acids, with a molecular weight of 17.2 kDa. Purity is typically greater than 97% and endotoxin level less than 1 EU/ug. The expected biological activity (ED50) is determined by the dose-dependent proliferation of mouse BALB/c 3T3 cells and is typically less than 1 ng/mL. The specific activity of this protein is 1x10^6 units/mg. Reconstitute using sterile water at 0.1 mg/ml and centrifuge prior to opening vial. Gently pipet solution down the sides of the vial. DO NOT VORTEX sample. Store reconstituted material at -20ºC and add 0.1% BSA for additional stability. Amino acid sequence: AAGSITTLPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHIK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTDEC FFFERLESNN YNTYRSRKYT SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS.