Fibroblast Growth Factor basic (human) Recombinant Protein, Invitrogen™
Fibroblast Growth Factor basic (human) Recombinant Protein, RP-8627, from Invitrogen™
Manufacturer: Pierce Biotechnology Inc RP8627
Fibroblast Growth Factor basic (human) Recombinant Protein, RP-8627, from Invitrogen.Fibroblast Growth Factors (FGFs) are a 22 member family of proteins known to be involved in angiogenesis, wound healing and embryonic development. As a family, they bind to heparin and signal through four receptor tyrosine kinases called, FGFR1, 2, 3 and 4. Although the mechanism remains unclear, FGF-basic 147 (variant of FGF basic 154), also called FGF-2, is a critical component in keeping embryonic stem cells undifferentiated in cell culture systems.
Host Species: E. Coli
Formulation: 50μg purified protein (0.1mg/mL) in 10mM sodium phosphate and no preservative; pH 8.0
Recombinant human FGF-b 147 (FGF-2) is a non-glycosylated protein, containing 147 amino acids, with a molecular weight of 16.5 kDa. Purity is typically greater than 97% and endotoxin level less than 1 EU/ug. The expected biological activity (ED50) is determined by the dose-dependent proliferation of mouse BALB/c 3T3 cells and is typically less than 1 ng/mL. The specific activity of this protein is 1x10^6 units/mg. Reconstitute using sterile water at 0.1 mg/ml and centrifuge prior to opening vial. Gently pipet solution down the sides of the vial. DO NOT VORTEX sample. Store reconstituted material at -20ºC and add 0.1% BSA for additional stability. Amino acid sequence: MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS.
|50μg purified protein (0.1mg/mL) in 10mM sodium phosphate and no preservative; pH 8.0|
|Human Fibroblast Growth Factor basic Recombinant Protein|
|store at -20°C, Avoid Freeze/Thaw Cycles|