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Thermo Scientific™ Pierce™ ECL 2 Western Blotting Substrate

Recommended HRP substrate for fluorescence imagers

Manufacturer: thermo scientific™  80196X3

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Catalog No. PI80196X3


Description & Specifications


Kit Contents Detection reagent 1 and 2
Product Type Pierce ECL 2 western blotting Substrate
Quantity 3 x 100mL
Sufficient For 30 Mini-gel blots, 3000cm2 of membrane
Storage Requirements 2° to 8°C

Pierce ECL 2 Substrate is a chemiluminescent and chemifluorescent substrate to detect peroxidase activity from HRP-conjugated antibodies and probes by Western blotting using X-ray film, CCD camera documentation systems or laser-based imagers. Pierce ECL 2 Substrate generates acridinium esters when it reacts with HRP. As these ester intermediates react with peroxide, they produce a strong and sustained chemiluminescent signal that can be captured by X-ray film CCD imaging. The substrate also produces a robust fluorescent signal that can be captured with fluorescence imagers.

Pierce ECL 2 substrate enables the detection of low-picogram amounts of target protein on nitrocellulose or PVDF membrane when probed with appropriate primary and secondary antibody concentrations. A broad dynamic range and excellent sensitivity combined with a long-lasting signal make Pierce ECL 2 Substrate a desirable Western blotting substrate.


  • High sensitivity — detect targets down to the low-picogram level
  • Long signal duration — sustained light output for as long as five hours
  • More imaging options — X-ray, CCD or laser-based imagers
  • More affordable — high quality and performance at a lower price than other suppliers' similar substrates