Uses high-affinity antibodies to efficiently isolate c-Myc-tagged proteins and interactors
$743.89 - $743.89
The c-Myc peptide (EQKLISEEDL) has become a popular fusion tag for mammalian recombinant protein expression, and this kit facilitates immunoprecipitation (IP) or co-immunoprecipitation (co-IP) experiments involving c-Myc-tagged proteins. The kit includes crosslinked beaded agarose to which a highly specific anti-c-Myc antibody is covalently immobilized. Upon incubation with a sample containing the tagged fusion protein, interaction complexes involving the c-Myc-tagged bait protein are captured on the agarose beads. After simple washing steps, the specific protein interaction complex is easily eluted from the resin in the supplied elution buffer or SDS-PAGE sample loading buffer for subsequent analysis. The kit includes the prepared agarose affinity resin, buffers, microcentrifuge spin columns, a positive control and easy-to-follow instructions.