For multiplex quantitative protein expression analysis by mass spectrometry
$127.05 - $9,366.00
Changes in protein expression and post-translational modifications are essential mechanisms of biological regulation and disease. Advancements in mass spectrometry (MS) instrumentation, bioinformatics and quantification methods, such as label-free quantification, metabolic labeling and chemical tagging, now enable researchers to identify and quantitatively analyze thousands of proteins in a given sample with a high degree of accuracy.1, 2Isobaric chemical tags are powerful tools that enable concurrent identification and quantitation of proteins in different samples using tandem mass spectrometry. They are small chemical molecules with identical structure that covalently attach to the free amino termini of lysine residues of peptides and proteins, thereby labeling various peptides in a given sample. During the MS/MS analysis, each isobaric tag produces a unique reporter ion signature that makes quantitation possible. In a typical MS analysis, the labeled peptides are indistinguishable from each other; however, in the tandem MS mode during which peptides are isolated and fragmented, each tag generates a unique reporter ion. Protein quantitation is then accomplished by comparing the intensities of the six reporter ions in the MS/MS spectra.
The tags consist of TMT0, the TMT2 two-plex set and the TMT6 six-plex set