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Thermo Scientific™ MagJET™ Plasmid DNA Kit


Purifies plasmid DNA from overnight E. coli cultures using paramagnetic bead technology.

Manufacturer: thermo scientific™  K2791

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Catalog No. FERK2791

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Description & Specifications

Specifications

For Use With (Application) High purity plasmid DNA suitable for all conventional molecular biology procedures like: FastDigest or conventional restriction digestion, Automated fluorescent and radioactive sequencing, PCR, In vitro transcription, Transformation, Transfection of robust cell lines
Includes Lysis Buffer, Resuspension Buffer, Neutralization Buffer, MagJET Magnetic Beads, RNase A, Wash Buffer 1 (conc.), Wash Buffer 2 (conc.), Elution Buffer
Quantity For 96 Preparations
Shelf Life 3 Years
Storage Requirements Once opened store RNase A solution at -20deg.C. MagJET Magnetic Beads should be stored at 4deg.C. Other components of the kit should be stored at room temperature (15deg. To 25deg.C).

The Thermo Scientific MagJET Plasmid DNA Kit is designed for fast and efficient purification of plasmid DNA (up to 4-5 μg/mL) or high copy plasmid DNA from overnight E. coli culture.
the kit utilizes paramagnetic bead technology enabling high yields and robust performance. High binding capacity, uniform particle size, and rapid magnetic response of MagJET Magnetic Beads makes the technology ideal for high throughput automatic nucleic acid purification, as well as for manual purification for low sample throughput users.
The resulting high quality DNA is free of proteins, nucleases and other contaminants or inhibitors. Purified plasmid DNA can be used in a wide range of downstream applications such as PCR, qPCR and other enzymatic reactions.
Highlights
High yields - up to 5 μg of plasmid DNA from 1 mL of bacterial culture
Pure - isolated DNA is immediately ready-to-use in downstream application such as sequencing, transcription, digestion with restriction enzymes or transfection of robust cell lines
Flexible - automation and manual processing protocols available
Repeatable results and robust performance
Applications
Fast isolation of high purity plasmid DNA suitable for all conventional molecular biology procedures, including:
• FastDigest or conventional restriction digestion
• Automated fluorescent and radioactive sequencing
• PCR
• In vitro transcription
• Transformation
• Transfection of robust cell lines
Includes
• Lysis Buffer
• Resuspension Buffer
• Neutralization Buffer
• MagJET Magnetic Beads
• RNase A
• Wash Buffer 1 (conc.)
• Wash Buffer 2 (conc.)
• Elution Buffer

Hazardous:Yes
> Hazardous::No
> Quality Control:Thermo Scientific MagJET Plasmid DNA Kit is qualified by isolating high copy plasmid DNA from 0.5-5 mL of overnight E. coli culture grown in LB or rich growth medium (OD600 = 2-6). The quality of isolated DNA is evaluated spectrophotometrically and by agarose gel electrophoresis. The purified DNA has an A260/A280 ratio of 1.8±0.2.
> Shelf Life:36 Months
> Shipping Condition:Ambient
> Storage Condition:Ambient