The Thermo Scientific MagJET Plant Genomic DNA Kit is designed for automated high throughput or manual purification of genomic DNA from a wide variety of plant species. The kit exploits an efficient magnetic particle technology eliminating need for phenol/chloroform extraction or alcohol precipitation.
The MagJET Plant Genomic DNA Kit procedures are optimized for the isolation of genomic DNA from 10-100 mg plant samples. DNA yields vary between different species and tissues depending on genome size, ploidy, cell number, and age of tissue sample.
The kit utilizes paramagnetic bead technology enabling high yields and robust performance. High binding capacity, uniform particle size, and rapid magnetic response of MagJET magnetic beads makes the technology ideal for high throughput automatic nucleic acid purification, as well as for manual purification for low sample throughput.
The resulting high quality DNA is free of proteins, nucleases and other contaminants or inhibitors, and can be used in a wide range of downstream applications such as PCR, qPCR or other enzymatic reactions. See Table 1 for typical genomic DNA yields from various sources.
- Versatile - suitable for any plant type and part, including woody, polyphenol-rich and lignified samples
- High gDNA yields compared to traditional CTAB method
- High quality - isolates high integrity gDNA, free of inhibitors and RNA contamination
- Flexible - easily adjustable for high throughput automation platforms and manual processing
Isolated genomic DNA is ideal for use in common molecular biology procedures, including:
- Lysis Buffer A
- Lysis Buffer B
- MagJET Magnetic Beads
- RNase A
- Precipitation Solution
- Wash Buffer 1 (conc.)
- Wash Buffer 2 (conc.)
- Elution Buffer
Quality Control: The MagJET Plant Genomic DNA Kit is qualified by isolating genomic DNA from 100 mg of plant tissue following the protocols outlined in the manual. The quality of isolated DNA is evaluated spectrophotometrically and by agarose gel electrophoresis. The purified DNA has an A260/A280 ratio of 1.8±0.3. A single band of more than 30 kb is observed after agarose gel electrophoresis and ethidium bromide staining. The functional quality of purified DNA is evaluated by digestion with restriction endonucleases.
Shelf Life: 36 Months
Shipping Condition: Ambient
Storage Condition:>/b> Ambient
|Isolated genomic DNA is ideal for use in common molecular biology procedures like PCR and qPCR, FastDigest and conventional restriction digestion, Southern blotting|
|Lysis Buffer A, Lysis Buffer B, MagJET Magnetic Beads, RNase A, Precipitation Solution, Wash Buffer 1 (conc.), Wash Buffer 2 (conc.), Elution Buffer|
|RNase A solution is stable at room temperature as long as the vial remains sealed. After being opened it should be stored at -20deg.C. MagJET Magnetic Beads should be stored at 4°C. Other components of the kit should be stored at room temperature (15deg. To 25deg.C).|