The MagJET mRNA Enrichment Kit is designed for fast and efficient purification of mRNA from total RNA samples. The oligo (dT) beads bind poly A+ RNA specifically and with high capacity, which ensures maximum extraction of mRNA from a wide range of starting material amount.
The kit utilizes paramagnetic bead technology enabling high yields and robust performance. High binding capacity, uniform particle size, and rapid magnetic response of MagJET oligo (dT) beads makes the technology ideal for high throughput automated nucleic acid purification, as well as for manual nucleic acid purification by low sample throughput users.
The MagJET mRNA Enrichment Kit protocol results in the highest amounts of intact full length RNA transcripts and low rRNA contamination.The resulting high quality purified mRNA is free of proteins, nucleases and other contaminants or inhibitors.
• Up to 10-fold mRNA enrichment
• Full length high integrity mRNA isolation
• High yields from broad (5 - 100 μg) RNA input range
• Compatible with high throughput automation
Isolated RNA transcripts can be used in:
• Hybridization Buffer for MagJET mRNA Enrichment Kit
• MagJET Oligo(dT) Beads
• Wash Buffer for MagJET mRNA Enrichment Kit
• Water, nuclease-free
> Quality Control:Thermo Scientific MagJET mRNA Enrichment Kit is qualified by isolating mRNA from 30 μg of total HeLa RNA following the protocol outlined in the manual. The quality of isolated mRNA is determined by measuring rRNA contamination using RT-qPCR. The amount of rRNA is <0.5% from starting material.
> Shelf Life:36 Months
> Shipping Condition:Ambient
> Storage Condition:4 C
|Isolated RNA transcripts can be used in: RT-PCR, RT-qPCR|
|Hybridization Buffer, MagJET Oligo(dT) Beads, Wash Buffer, nuclease-free water|
|Store at 4deg.C.|