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Thermo Scientific™ Luminaris HiGreen qPCR Master Mix, High ROX

Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR.

Manufacturer:  Thermo Scientific™ K0961

 View more versions of this product

Catalog No. FERK0961


 

Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Includes:
  • Luminaris HiGreen High ROX qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer. The master mix contains high concentration of ROX passive reference dye for specific real-

Specifications

Description

PCR Reagent, Real-Time; Luminaris HiGreen qPCR Master Mix, High ROX; 250 Reactions; For use with: 7000 System, 7300 System, 7700 System, 7900HT System, StepOne System, StepOnePlus System; Sample type: DNA, dsDNA

  • UDG in the master mix to prevent carry-over contamination
  • Equal performance of blue and colorless master mixes
  • Versions with premixed ROX or fluorescein for different real-time platforms
  • Standard cycling protocols
  • Excellent qPCR performance
  • Specificity: Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
  • Sensitivity: ingle copy detection
  • Wide linear range: accurate quantification across 9 orders of magnitude
  • Reproducibility and convenience: ready-to-use 2X master mix
Description & Specifications

Specifications

Detection Method: SYBR, PCR Method: qPCR
7000 System, 7300 System, 7700 System, 7900HT System, StepOne™ System, StepOnePlus™ System
250 Reactions
DNA, dsDNA
2X
Real time PCR (qPCR), gene expression analysis, siRNA validation, genotyping and pathogen detection
Luminaris HiGreen High ROX qPCR Master Mix (2X) includes Hot Start Taq DNA Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer. The master mix contains high concentration of ROX passive reference dye for specific real-time platforms and is supplied with nuclease-free water.
250 x 20μL reactions
Store at -20°C.