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Thermo Scientific™ Luminaris HiGreen Fluorescein qPCR Master Mixes


Ready-to-use qPCR master mixes with premixed UDG optimized for SYBR Green chemistry using standard cycling protocol. Master mixes available with or without blue color.

Manufacturer: thermo scientific™  K0982

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Catalog No. FERK0982

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Description & Specifications

Specifications

Concentration 2X
For Use With (Application) Gene expression analysis, siRNA validation, Genotyping, Pathogen detection
Includes 2x Luminaris HiGreen Fluorescein qPCR Master Mix (4 x 1.25mL): includes Hot Start Taq DNA Polymerase, UDG and dNTPs (also dUTP) in an optimized colorless PCR buffer, supplied with nuclease-free water (4 x 1.25mL). Also contains fluorescein passive reference dye for specific real-time platforms.
Quantity 500 x 20μL rxns
Shelf Life 2 Years
Storage Requirements Store at -20°C

Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.
The Hot Start Taq DNA polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control (see Reference 1). The Luminaris Color HiGreen qPCR Master Mixes are supplemented with an inert blue dye and a separate Yellow Sample Buffer that contains a yellow dye. The qPCR reaction mix containing both components is green.
The use of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes with ROX at different concentrations or fluorescein as passive reference dyes are available for most qPCR platforms (see Real-time PCR instrument compatibility chart).

Highlights

  • Blue master mix and Yellow Sample Buffer for easy pipetting
  • UDG in the master mix to prevent carry-over contamination
  • Excellent qPCR performance
  • Specificity - Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
  • Sensitivity - single copy detection
  • Wide linear range - accurate quantification across 9 orders of magnitude
  • Reproducibility and convenience - ready-to-use 2X master mix
  • Equal performance of blue and colorless master mixes
  • Versions with premixed ROX or fluorescein for different real-time platforms
  • Standard cycling protocols
Applications
  • Gene expression analysis
  • siRNA validation
  • Genotyping
  • Pathogen detection
Includes
  • Luminaris Color HiGreen qPCR Master Mixes include Hot Start Taq DNA Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer with a blue dye; supplied with 40X Yellow Sample Buffer and nuclease-free water.
  • Luminaris Color HiGreen High ROX qPCR Master Mix (2X) and Luminaris Color HiGreen Low ROX qPCR Master Mix (2X) contain different amount of ROX passive reference dye for specific real-time platforms (see Real-Time PCR instrument compatibility chart).
  • Luminaris Color HiGreen Fluorescein qPCR Master Mix (2X) contains fluorescein passive reference dye for specific real-time platforms (see Real-Time PCR instrument compatibility chart).
  • Luminaris HiGreen qPCR Master Mixes include Hot Start Taq DNA Polymerase, UDG and dNTPs (also dUTP) in an optimized colorless PCR buffer; supplied with nuclease-free water.
  • Luminaris HiGreen High ROX qPCR Master Mix (2X) and Luminaris HiGreen Low ROX qPCR Master Mix (2X) contain different amount of ROX passive reference dye for specific real-time platforms (see Real-Time PCR instrument compatibility chart).
  • Luminaris Color HiGreen Fluorescein qPCR Master Mix (2X) contains fluorescein passive reference dye for specific real-time platforms (see Real-Time PCR instrument compatibility chart).

Hazardous: No
Shelf Life: 24 Months
Shipping Condition: Frozen Gel Packs
Storage Condition: −20°C
Storage Notes: Note that SYBR Green and ROX dyes are light sensitive. Exposure to light should be minimized.
References:M. C. Longo et al., Gene 93, 125-128 (1990).