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Thermo Scientific™ Luminaris™ Color HRM Master Mix


Ready-to-use master mix for easy and accurate discrimination of DNA sequence variants by High-Resolution Melting (HRM) analysis.

Manufacturer: thermo scientific™  K1031

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Catalog No. FERK1031

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Description & Specifications

Specifications

Concentration 2X
For Use With (Application) SNP genotyping, Mutation discovery (gene scanning), Heterozygosity screening, Bacterial genotyping, CpG methylation analysis
Includes Luminaris Color HRM Master Mix (2X) includes chemically modified Hot Start Taq DNA Polymerase, EvaGreen™ fluorescent dye, dNTPs and MgCl2 in an optimized PCR buffer with a blue dye; 40x Yellow Sample Buffer with a yellow dye; Nuclease-free water
Quantity 250 x 20μL reactions
Shelf Life 2 Years
Storage Requirements Store at -20deg.C in dark for long term storage or at 4deg.C for up to three weeks. When stored at -20deg.C, full activity of the mix is retained for at least 24 months as indicated on the tube label. Repeated freezing and thawing of the Master Mix is not recommended.

Thermo Scientific Luminaris Color HRM Master Mix is a convenient, ready-to-use master mix designed for the accurate detection of DNA sequence variations using High Resolution Melt (HRM) analysis method. The Master Mix complements Thermo Scientific PikoReal Real-Time System and software to provide a complete solution for HRM analysis.
The Luminaris Color HRM Master Mix contains Hot Start Taq DNA polymerase in an optimized buffer system with EvaGreen™ fluorescent dye for robust and reliable performance in PCR and HRM. The chemically modified Hot Start Taq DNA polymerase has ultra low residual activity until thermally activated ensuring PCR specificity and sensitivity. The DNA binding dye EvaGreen™ enables discrimination of even the most challenging sequence differences (such as Type IV SNP) without PCR inhibition and with no apparent sequence preference. The Luminaris Color HRM Master Mix is supplemented with an inert blue dye and a separate Yellow Sample Buffer that contains a yellow dye. Mixing both components in a reaction turns the solution green. This provides a visual aid when pipetting and decreases the risk of errors during reaction setup, especially when using white reaction vessels.
Highlights
• High performance on most real-time instruments with HRM capabilities
• Dual-color system for easy reaction setup
• Superior discrimination of all four SNP classes
• High sensitivity - accurate results from 30 copies of genomic DNA
• Exceptional stability of fully assembled reaction - for high-throughput applications
• Optimized for PikoReal Real-Time System and software
Applications
• SNP genotyping
• Mutation discovery (gene scanning)
• Heterozygosity screening
• Bacterial genotyping
• CpG methylation analysis
Includes
• Luminaris Color HRM Master Mix (2X) includes chemically modified Hot Start Taq DNA Polymerase, EvaGreen™ fluorescent dye, dNTPs and MgCl2 in an optimized PCR buffer with a blue dye.
• 40x Yellow Sample Buffer with a yellow dye.
• Nuclease-free water.

Hazardous:No
> Shelf Life:24 Months
> Shipping Condition:Frozen Gel Packs
> Storage Condition:-20°C
> Storage notes:Exposure to light should be minimized to avoid the loss of fluorescent signal intensity.