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TER-119 Rat anti-Mouse, PE, Clone: TER-119, eBioscience™

Rat Monoclonal Antibody

Manufacturer:  Life Technologies LS12592182

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Catalog No. 501129611



Description

Description

Description: The TER-119 monoclonal antibody reacts with mouse erythroid cells from early proerythroblast to mature erythrocyte stages. The TER-119 antigen is present in yolk sac, fetal and newborn liver, but is not expressed by cells carrying BFU-E and CFU-E activities. Several erythroleukemia cell lines tested so far are negative for expression of TER-119 antigen even after dimethylsulfoxide stimulation. Biochemical and molecular analysis of the TER-119 antigen indicate that this molecule is associated with the surface glycophorin A, but is not a typical glycophorin. Applications Reported: The TER-119 antibody has been reported for use in flow cytometric analysis. Applications Tested: The TER-119 antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.

TER-119 is a lineage marker for erythroid cells from early proerythroblast to mature erythrocyte stages in adult blood, spleen, and bone marrow. It is also present in yolk sac, and fetal and newborn liver. The TER-119 antigen is not expressed by cells of earlier erythroid development at BFU-e (blast-forming unit erythroid) stage or CFU-e (colony-forming unit erythroid) stage.
Specifications

Specifications

TER-119
Monoclonal
0.2 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Liquid
Ly76
IgG2b, kappa
100 μg
4° C, store in dark, DO NOT FREEZE!
Primary
104231
Flow Cytometry
TER-119
PE
Ly76
TER119; Erythroid cell marker; Ly-76; Ly76
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
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