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Cytiva TempliPhi™ Sequence Resolver Kit

Uses modified nucleotides in isothermal rolling circle amplification (RCA) to improve results with difficult to sequence templates

Manufacturer:  Cytiva 28903529

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Catalog No. 45-002-402

Includes: Sample buffer, Reaction buffer and Enzyme mix



  • Cultureless preparation from bacterial colonies, glycerol stock or purified DNA, saves time and reagents
  • Requires 20 minutes hands-on time
  • Excellent sequence finishing performance resolving sequencing stop and compression issues associated with difficult templates
  • Improve difficult template sequencing success with up to 820 bp Phred 20 read length
  • For use directly in cycle sequencing reactions without purification
  • Available in sizes of 20, 50 and 500 reactions



Store the kit at -70°C. The enzyme mix must be stored at -70°C; all other components may be stored at -20°CThaw components on ice and maintain at 0°C to 4°C during handling
Amplification of difficult templates for successful DNA sequencing
18 h
illustra TempliPhi Sequence Resolver Kit uses the highly processive, strand-displacing Phi29 DNA polymerase and modified nucleotides to amplify circular templates for subsequent sequencing.
1 x 90μL Sample Buffer, 1 x 90μL Reaction Buffer, 1 x 90μL Enzyme mix
illustra TempliPhi Sequence Resolver Kit
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