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Thermo Scientific™ Taq DNA Polymerase, native, without BSA (5 U/µL)

Optimize amplification of DNA samples of low purity with this highly thermostable, native DNA polymerase for routine PCR.

Manufacturer:  Thermo Scientific™ EP0282

Catalog No. FEREP0282



Description

Description

Native Taq DNA Polymerase is a highly thermostable DNA polymerase of the thermophilic bacterium Thermus aquaticus. The enzyme catalyzes 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, the polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Native Taq DNA Polymerase is preferred for amplification of bacterial DNA sequences homologous to those found in E. coli.

Taq DNA Polymerase (native, with BSA) is supplied with BSA as a stabilizing agent. This version of Taq DNA Polymerase is often the best choice when amplifying DNA samples of lower purity, e.g. genomic DNA from mouse tail.

Highlights

  • Thermostable -half life is more than 40 min at 95°C.
  • Generates PCR products with 3'-dA overhangs.
  • Supplied with two buffers -10X Taq Buffer with KCl and 10X Taq Buffer with (NH4)2SO4. The latter allows for PCR at wide range of magnesium concentrations and decreases unspecific priming.
  • Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides).
Specifications

Specifications

Contains:
• 100 µL Taq DNA Polymerase, native (5 U/µL)
• 2 x 1.25 mL 10X Taq Buffer with KCl
• 2 x 1.25 mL 10X Taq Buffer with (NH4)2SO4
• 2 x 1.25 mL 25 mM MgCl2

Store at -20°C.
Primer-Probe
Low
3'-A
Standalone
DNA
2 x 1 mL of 25 mM MgCl2, 2 x 1 mL of 10X Taq Buffer with KCl, 2 x 1 mL of 10X Taq Buffer with (NH4)2SO4
PCR Amplification
10X
1 X
No
Taq DNA Polymerase
Standard
2 mL
Taq DNA Polymerase
SDS
Documents
Product Certifications
Promotions

Promotions

In certain countries use of this product is covered by patents. Purchase of product in these countries includes non-transferable, limited license for using only this amount of product for the purchaser's own internal research.