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Thermo Scientific™ T4 DNA Polymerase

Ensure accuracy in DNA synthesis with template-dependent T4 DNA Polymerase that catalyzes the 5'-3' synthesis from primed single-stranded DNA.

Manufacturer: thermo scientific™  EP0062

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Catalog No. FEREP0062


Description & Specifications


For Use With (Application) Blunting of DNA ends: fill-in 5'-overhangs or/and removal of 3'-overhangs (1, 2); Blunting of PCR products with 3'-dA overhangs; Synthesis of label
Includes 2 x 1mL of 5X Reaction Buffer
Product Name T4 DNA Polymerase
Quantity 500U

T4 DNA Polymerase, a template-dependent DNA polymerase, catalyzes 5'-3' synthesis from primed single-stranded DNA. The enzyme has a 3'-5' exonuclease activity, but lacks 5'-3' exonuclease activity.


  • Stronger 3'-5' exonuclease activity on single-stranded than on double-stranded DNA and greater (more than 200 times) than DNA polymerase I, E. coli,and Klenow fragment
  • Active in Thermo Scientific restriction enzyme, PCR, RT and T4 DNA Ligase buffers