Thermo Scientific™ SuperSignal™ ELISA Femto Maximum Sensitivity Substrate
HRP substrate formulated for superior protein detection and low-end linearity in chemiluminescent ELISA applications.
Manufacturer: Thermo Scientific™ 37074
Thermo Scientific™ SuperSignal ELISA Femto Maximum Sensitivity Substrate is formulated for superior protein detection and low-end linearity in chemiluminescent ELISA applications.SuperSignal ELISA Femto Substrate generates detectable chemiluminescence (light) within one minute of addition to trace amounts of soluble HRP, saving up to 30 minutes per assay compared to colorimetric ELISA substrates. This feature is ideal for high-throughput screening (HTS) applications in which as many as 100,000 assays can be performed daily on robotic equipment. Because incubation periods are often a limiting factor in the development of rapid automated assays, SuperSignal ELISA Femto Substrate is the logical choice for automated HTS applications.
Immediate light generation – intense signal generated immediately at both room temperature and 37°C
Improved low-end linearity – easy detection of low quantities of proteins with high signal-to-noise ratios and low-end linearity of dose response curves
High sensitivity – femtogram-level detection of target proteins
Reduction in assay time – high sensitivity allows for reduction in incubation steps
Stability – storage for six months at room temperature or a minimum of 12 months at 4°C with a six-hour working solution stability
Emits light at 425nm – measurable by luminometers
Luminol/Enhancer Solution; Peroxide Buffer
|SuperSignal ELISA Femto Substrate|
|Stable Peroxide Substrate, 125mL, Luminol/Enhancer, 125mL|
|250mL of 1X substrate (15 to 25 microplate assays)|
|Proprietary luminol and peroxide solutions|
|4°C for up to one year or at room temperature for up to six months.|