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Thermo Scientific™ SuperBlock™ Blocking Buffers

Block membranes, plates and tissue samples with these optimized solutions containing a protein that provides fast and effective blocking for Western blot, ELISA and IHC.

Manufacturer:  Thermo Scientific™ PI37537

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Catalog No. PI37537



Thermo Scientific™ SuperBlock Blocking Buffers are optimized PBS and TBS solutions containing a protein that provides incredibly fast and effective blocking for ELISA, immunohistochemistry and Western blotting applications.

Blocking microplates, membranes or tissues with a SuperBlock Buffer yields a high signal-to-noise ratio in most detection systems. The protein-based formulation does not contain any immunoglobulins, albumin or endogenous biotin, making it compatible in many situations where traditional blocking agents fail. The buffer is especially effective at blocking coated polystyrene microplates (96-well plates) and stabilizing them for drying and storage for later use.


  • Fast – block membranes in 5 to 10 minutes and ELISA plates in 2 minutes

  • Flexible – guaranteed to be biotin-free for use with streptavidin system

  • Convenience – available in multiple buffer formats (in PBS or TBS with or without Tween™-20 detergent) and package sizes (1L, 5L and dry-blend pouches)

  • Low background – the non-serum protein solution yields a high signal-to-noise ratio

  • Stable – store buffer at 4°C for one year; store blocked plates dry for up to 12 months



SuperBlock (TBS) Blocking Buffer for Blotting
Store dry blend at room temperature; Store at 4°C after reconstitution
Proprietary protein formulation in Tris-buffered saline, pH 7.4, with Kathon preservative
30 blots at 30mL each