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Molecular Probes™ Streptavidin, Alexa Fluor™ 647 conjugate DFS Item

Streptavidin, Alexa Fluor™ 647 conjugate

Manufacturer:  Molecular Probes™ LSS32357

 View more versions of this product

Catalog No. S32357



Description

Description

Alexa Fluor™ 647 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor™ dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays. Alexa Fluor™ dye streptavidin conjugates are supplied as 1 mg lyophilized product or in 0.5 mL volumes of a 2 mg/mL solution.

Important Features of Alexa Fluor™ 647 Streptavidin Conjugates:
  • Alexa Fluor™ 647 streptavidin conjugate has Ex/Em maxima of ~ (650/668)
  • Bright, photostable fluorescence
  • High solubility in aqueous solutions
  • Available in multiple colors
  • Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more

    Properties of Alexa Fluor™ Dyes
    Alexa Fluor™ dyes are organic fluorescent dyes developed for better performance in imaging and other labeling protocols and exhibit improved photostability and brightness and improved solubility in aqueous solutions. Available in a broad range of colors, these dyes are a good choice for most imaging applications.

    Blocking Endogenous Biotin
    Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.

    For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

    Related Links:

    Learn more about Avidin-Biotin Detection

    Learn more about Alexa Fluor™ Dyes

    Find out about other Labeled Streptavidin Conjugates

    Read Avidin and Streptavidin Conjugates-Section 7.6 in the Molecular Probes Handbook
  • Specifications

    Specifications

    Very high binding affinity for biotin
    2mg/mL
    Alexa Fluor™ 647 Conjugate Streptavidin, 0.5mL, Blue, Liquid Form, 2mg/mL Concentration, Alexa Fluor™ 647 Label or Dye, Used in Western Blots, Flow Cytometry, Imaging and Microscopy, Microplate Assays, 2 to 8°C Storage Temperature
    Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more
    Liquid
    7.2
    Alexa Fluor™ 647 Conjugate Streptavidin
    0.5mL
    0.5mL
    2 to 8°C
    1 Each
    Blue
    Alexa Fluor™ 647
    Fluorescent
    Lyophilized
    0.5mL
    Alexa Fluor™ 647 Conjugate Streptavidin
    TLC
    For research use only
    0.5mL
    0.5mL
    2mg/mL solution in PBS, pH 7.2, 5mM Sodium azide
    Documents

    For Research Use Only. Not for use in diagnostic procedures.