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SPHK2 Recombinant Human Protein DFS Item

SPHK2 Recombinant Human Protein

Manufacturer:  Life Technologies LSPV5216

Catalog No. PV5216



Description

Description

SPHK2, along with SPHK1, catalyzes the phosphorylation of sphingosine producing the signalling molecule sphingosine-1-phosphate (S1P). S1P signals through G-protein coupled receptors and controls diverse cellular processes. Inhibitors of SPHKs are viewed as potential therapuetics for the treatment of cancer.
Specifications

Specifications

NP_064511.2
Pharma and biopharma, protein assays and analysis, target and lead identification and validation
SPHK2 Recombinant Human Protein
20μg
For research use only. Not for use in diagnostic procedures
Lipid kinase
SPHK2 Recombinant Human Protein
Kinase assay
Baculovirus
SPHK2 Recombinant Human Protein
Human
0.39mg/mL
96.7kDa
90% by SDS-PAGE
–80°C
1 Each
SPHK2
SPHK2 was diluted in assay buffer and the activity determined in a 10μL Adapta™ assay (PV5099) with 10μM ATP and 50μM Sphingosine kinase substrate (PV5372) at room temperature for 1 hour. The reaction was stopped with a 5μL addition of EDTA and detection reagents. The final concentrations are 10mM EDTA, 2nM Adapta™ Eu-anti-ADP antibody and 6nM Alexa Fluor™ 647 ADP Tracer. The plate was read after a 45 min. room temperature incubation
Kinase and inhibitors
SPHK and DGCK
170nmoles of ADP formed per minute per mg of total protein using the Adapta™ assay (PV5099). An ADP/ATP standard curve is used to calculate percent conversion of ATP to ADP. SPHK2 activity determined at a final protein concentration of 78.1ng/mL
GST Tag
Documents

For Research Use Only. Not for use in diagnostic procedures.