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SPHK1 Recombinant Human Protein DFS Item

SPHK1 Recombinant Human Protein

Manufacturer:  Life Technologies LSPV5214

Catalog No. PV5214



Description

Description

SPHK1, along with SPHK2, catalyzes the phosphorylation of sphingosine producing the signalling molecule sphingosine-1-phosphate (S1P). S1P signals through G-protein coupled receptors and controls diverse cellular processes. Inhibitors of SPHKs are viewed as potential therapuetics for the treatment of cancer.
Specifications

Specifications

NP_068807.2
Pharma and biopharma, protein assays and analysis, target and lead identification and validation
SPHK1 Recombinant Human Protein
20μg
For research use only. Not for use in diagnostic procedures
Lipid kinase
SPHK1 Recombinant Human Protein
Kinase assay
Baculovirus
SPHK1 Recombinant Human Protein
Human
0.37mg/mL
71.4kDa
95% by SDS-PAGE
–80°C
1 Each
SPHK1
SPHK1 was diluted in assay buffer and the activity determined in a 10μL Adapta™ assay (PV5099) with 1μM ATP and 50μM Sphingosine kinase substrate (PV5372) at room temperature for 1 hour. The reaction was stopped with a 5μL addition of EDTA and detection reagents. The final concentrations are 10mM EDTA, 2nM Adapta™ Eu-anti-ADP antibody and 1.6nM Alexa Fluor™ 647 ADP Tracer. The plate was read after a 45 min. room temperature incubation
Kinase and inhibitors
SPHK and DGCK
1,040nmoles of ADP formed per minute per mg of total protein using the Adapta™ assay (PV5099). An ADP/ATP standard curve is used to calculate percent conversion of ATP to ADP. SPHK1 activity determined at a final protein concentration of 0.977ng/mL
GST Tag
Documents

For Research Use Only. Not for use in diagnostic procedures.