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Thermo Scientific™ Spectra™ Multicolor High Range Protein Ladder


A mixture of eight blue-, green- and orange-stained proteins (40 to 300kDa) for use as size standards for high MW proteins in gel electrophoresis and Western blotting.

Manufacturer: thermo scientific™  26625

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Catalog No. PI26625

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Description & Specifications

Specifications

Description Spectra Multicolor High Range Protein Ladder
Detection Visible
Formulation 8 proteins from 40 to 300kDa in reducing sample buffer
Quantity 2 × 250mL
Separation Range High
Stained Prestained
Sufficient For 50 to 100 mini gel lanes at 10 to 20μL per lane

Thermo Scientific™ Spectra Multicolor High Range Protein Ladder is a mixture of eight (8) blue-, green and orange-stained proteins (40 to 300kDa) for use as size standards for high MW proteins in gel electrophoresis and Western blotting.

This prestained protein MW marker is designed for monitoring the progress of SDS-polyacrylamide gel electrophoresis, for assessing transfer efficiency onto PVDF, nylon and nitrocellulose membranes, and for estimating the approximate size of separated proteins that have been made visible with gel stains or Western blot detection reagents. Three different chromophores (blue, orange, green) are bound to the different component proteins, producing a brightly colored ladder with an easy-to-remember pattern. The Spectra Multicolor High Range Protein Ladder is ready to use: no heating, further dilution or addition of a reducing agent is required.

Highlights:

  • Size range – eight proteins spanning 40 to 300kDa

  • Multicolor – three different colors for unambiguous band-size assignment

  • Ready-to-use – supplied in a loading buffer for direct loading on gels; no need to boil

  • Sharp bands – color-coded bands of similar intensity for easy visualization

  • Quality tested – each lot evaluated by SDS-PAGE and Western blotting

  • Membrane-compatible – colored bands transfer to membranes for Western blotting

Includes:

Dye-stained proteins in 62.5mM Tris-H3PO4 (pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 10mM DTT, 1mM NaN3 and 33% glycerol.

Recommended for:

Monitoring of protein migration during SDS-PAGE; Verifying Western transfer efficiency; Approximate sizing of proteins on SDS-polyacrylamide gels and Western blots; Locating a protein of interest for excision from an unstained preparative gel