SOX2 Rat anti-Human, Mouse, eFluor® 660, Clone: Btjce, eBioscience™
Rat Monoclonal Antibody
Manufacturer: Life Technologies LS50981182
Description: The Btjce monoclonal antibody reacts with the transcription factor Sox2, a member of the SOX (sex determining region Y -related HMG (High Mobility Group) Box) family of proteins. Sox family members play a role in early organ development, andApplications Reported: This btjce antibody has been reported for use in intracellular staining followed by flow cytometric analysis and immunocytochemistry. Applications Tested: This btjce antibody has been tested by immunocyochemstry of fixed and permeabilized F9 cells. This can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. This btjce has also been tested by intracellular staining and flow cytometric analysis using the Foxp3/Transcription Factor Buffer and protocol. Please refer to intracellular Staining Protocol on Best Protocol web page - Protocol B: One-step protocol for intracellular (nuclear) proteins. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered. SOX2 is an intronless gene encoding a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of the SOX2 gene is required for stem-cell maintece in the central nervous system, and also regulates gene expression in the stomach. The SOX2 gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). Further, SOX2 protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation, optic nerve hypoplasia and syndromic microphthalmia.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|ANOP3, MCOPS3, SRY-related HMG-box gene 2, transcription factor SOX-2, transcription factor SOX2, sex determining region Y box 2|
|Flow Cytometry, Immunocytochemistry, Immunofluorescence|
|4° C, store in dark, DO NOT FREEZE!|
For Research Use Only.