SOX2, clone: Btjce, eBioscience™
Rat Monoclonal Antibody
Manufacturer: Life Technologies LS14981182
This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT).Applications Reported: This Btjce antibody has been reported for use in intracellular staining followed by flow cytometric analysis, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, immunocytochemistry, western blot and ELISA (Fluorochrome-conjugated Btjce is recommended for use in intracellular flow cytometry). Applications Tested: This Btjce antibody has been tested by immunohistochemistry on FFPE human testes at less than or equal to 5 μg/mL. The antibody can be used with either high or low pH antigen retrieval. This Btjce antibody has been tested by immunocytochemistry on fixed and permeabilized iPS cells at less than or equal to 5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered. SOX2 is an intronless gene encoding a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of the SOX2 gene is required for stem-cell maintece in the central nervous system, and also regulates gene expression in the stomach. The SOX2 gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). Further, SOX2 protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation, optic nerve hypoplasia and syndromic microphthalmia.
|PBS with 0.09% sodium azide; pH 7.2|
|ANOP3, MCOPS3, SRY-related HMG-box gene 2, transcription factor SOX-2, transcription factor SOX2, sex determining region Y box 2|
|ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot|
For Research Use Only.