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Sino Biological™ TNFSF10 Recombinant Human Protein DFS Item


This recombinant protein was expressed from a DNA sequence encoding the human TNFSF10 (NP_003801.1) (Val 114-Gly 281) fused to an initial Met

Manufacturer: life technologies  LS10409HNAE25

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 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Catalog No. 10409HNAE25

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Description & Specifications

Specifications

Storage Requirements Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Purity ≥95% by SDS-PAGE
Common Name Tumor necrosis (ligand) receptor superfamily, member 10
Protein Family Cytokines & Receptors/CD Proteins
Quantity 5 x 5μg
Gene Symbol TNFSF10
Protein Subtype TNF (Tumor Necrosis Factor)
Gene Alias APO2L, Apo-2L, CD253, TL2, TRAIL
Protein Tag Untagged
Validated Application Assay Standard
Expression System E. coli
Molecular Weight 19.6kDa
Format Lyophilized

Tumor Necrosis (Ligand) Receptor Superfamily Member 10 (TNFSF10), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.

  • Activity: 1. Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D, the ED50 for this effect is typically 4-8ng/mL; 2. Measured in a cytotoxicity assay using NCI-H460 cells in the presence of the metabolic inhibitor actinomycin D, the ED50 for this effect is typically 0.2-0.8ng/mL
  • Formulation: Lyophilized in 40mM Tris 0.3M NaCl pH 7.0, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.