DescriptionTumor necrosis factor receptor superfamily member 1A (TNFRSF1A/CD120a), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, studying protein-protein interactions, and cell biology research applications. It can also be used as an immunogen, or as a protein standard.
- Activity: 1. Measured by its binding ability in a functional ELISA; a. Immobilized human TNFa at 10μg/mL (100μL/well) can bind biotinylated human TNFRSF1A-his, the ED50 of biotinylated human TNFRSF1A-his is 0.04μg/mL; b. Immobilized mouse TNFa at 10μg/mL (100μL/well) can bind biotinylated human TNFRSF1A-his, the ED50 of biotinylated human TNFRSF1A-his is 0.28μg/mL; 2. Measured by its ability to inhibit the TNF mediated cytotoxicity in the L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D, the ED50 for this effect is typically 0.04-0.2μg/mL in the presence of 0.25ng/mL recombinant human TNF
- Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80
- Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible
Shipping Condition: Room Temperature. This protein is manufactured by Sino Biological Inc.
|≥90% by SDS-PAGE|
|CD120a, FPF, mgC19588, TBP1, TNF-R, TNF-R-I, TNF-R55, TNFAR, TNFR60, p55, p55-R, p60|
For Research Use Only. Not for use in diagnostic procedures.