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Sino Biological™ TNFRSF1A (CD120a) Recombinant Human Protein, His Tag DFS Item

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Thr 211) of human TNFRSF1A (NP_001056.1) fused to a polyhistidine tag at the C-terminus.

Manufacturer:  Life Technologies LS10872H08H50

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Catalog No. 10872H08H50


 

Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Specifications

Description

Protein; Sino Biological; TNFRSF1A (CD120a) Recombinant Human Protein, His Tag; 50ug; Source: Human; Purity: greater than or equal to 90% by SDS-PAGE

Tumor necrosis factor receptor superfamily member 1A (TNFRSF1A/CD120a), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, studying protein-protein interactions, and cell biology research applications. It can also be used as an immunogen, or as a protein standard.
  • Activity: 1. Measured by its binding ability in a functional ELISA; a. Immobilized human TNFa at 10μg/mL (100μL/well) can bind biotinylated human TNFRSF1A-his, the ED50 of biotinylated human TNFRSF1A-his is 0.04μg/mL; b. Immobilized mouse TNFa at 10μg/mL (100μL/well) can bind biotinylated human TNFRSF1A-his, the ED50 of biotinylated human TNFRSF1A-his is 0.28μg/mL; 2. Measured by its ability to inhibit the TNF mediated cytotoxicity in the L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D, the ED50 for this effect is typically 0.04-0.2μg/mL in the presence of 0.25ng/mL recombinant human TNF
  • Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

Shipping Condition: Room Temperature. This protein is manufactured by Sino Biological Inc.

Description & Specifications

Specifications

Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
7132
≥90% by SDS-PAGE
Store at -20° to -70°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
TNF (Tumor Necrosis Factor)
CD120a, FPF, mgC19588, TBP1, TNF-R, TNF-R-I, TNF-R55, TNFAR, TNFR60, p55, p55-R, p60
TNFRSF1A
Cytokines & Receptors/CD Proteins
His Tag
Lyophilized
22.7kDa
50μg
Truncated/Recombinant
<1 EU/μg
Tumor necrosis factor receptor superfamily, member 1A
Assay Standard
Mammalian