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Sino Biological™ TFPI Recombinant Human Protein, His Tag DFS Item

This recombinant protein was expressed from a DNA sequence encoding the human TFPI (NP_006278.1) (Met 1-Lys 282) fused to a C-terminal polyhistidine tag

$223.65 - $1,155.00

Specifications

Purity >85% by SDS-PAGE
Endotoxin Concentration <1 EU/μg
Format Lyophilized
Gene Alias EPI, LACI, TFI, TFPI1
View More Specs

 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

10564H08H5

DFS Item
 
life technologies
LS10564H08H5
5μg Each for $223.65

10564H08H50

DFS Item
 
life technologies
LS10564H08H50
50μg Each for $1,155.00
Description & Specifications

Specifications

Purity >85% by SDS-PAGE
Endotoxin Concentration <1 EU/μg
Format Lyophilized
Gene Alias EPI, LACI, TFI, TFPI1

Tissue Factor Pathway Inhibitor (Liporeotein-Associated Coagulation Inhibitor) (TFPI), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as enzymatic analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

  • Activity: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Anaspec Cat. No.27114), the IC50 value is <0.4nM, as measured in 100μL reaction mixture containing 1.25ng trypsin (Sigma Cat. No.T4799), 10μM substrate, 50mM Tris, 10mM CaCl2, 0.15M NaCl, 0.05% Brij-35, pH 7.5
  • Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.