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Sino Biological™ SPINT1 Recombinant Human Protein, His Tag DFS Item


This recombinant protein was expressed from a DNA sequence encoding the human SPINT1 (O43278-2) (Met 1-Val 433) fused to a polyhistidine tag at the C-terminus

Manufacturer: life technologies  LS11742H08H5

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 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Catalog No. 11742H08H5

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Description & Specifications

Specifications

Storage Requirements Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Gene Alias SPINT1, UNQ223/PRO256, HAI, HAI1, MANSC2
Quantity 5μg
Protein Tag His Tag
Protein Family Protease Inhibitors
Format Lyophilized
Protein Subtype Protease Inhibitors
Expression System Mammalian
Validated Application Assay Standard
Common Name Serine peptidase inhibitor, Kazal type 1
Gene Symbol SPINT1
For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Endotoxin Concentration <1 EU/μg
Molecular Weight 45.8kDa
Purity ≥90% by SDS-PAGE

Serine Peptidase Inhibitor Kazal Type 1 (SPINT1), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as enzymatic analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

  • Activity: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (R&D Systems Cat. No. ES002); the IC50 value is <2nM
  • Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.