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Sino Biological™ SPINT1 Recombinant Human Protein, His Tag DFS Item

This recombinant protein was expressed from a DNA sequence encoding the human SPINT1 (O43278-2) (Met 1-Val 433) fused to a polyhistidine tag at the C-terminus

Manufacturer: life technologies  LS11742H08H25

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For Research Use Only. Not for use in diagnostic procedures.

Catalog No. 11742H08H25


Description & Specifications


For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Format Lyophilized
Molecular Weight 45.8kDa
Purity ≥90% by SDS-PAGE
Quantity 5 x 5μg
Storage Requirements Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Protein Subtype Protease Inhibitors
Endotoxin Concentration <1 EU/μg
Gene Alias SPINT1, UNQ223/PRO256, HAI, HAI1, MANSC2
Common Name Serine peptidase inhibitor, Kazal type 1
Gene Symbol SPINT1
Protein Tag His Tag
Validated Application Assay Standard
Protein Family Protease Inhibitors
Expression System Mammalian

Serine Peptidase Inhibitor Kazal Type 1 (SPINT1), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as enzymatic analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

  • Activity: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (R&D Systems Cat. No. ES002); the IC50 value is <2nM
  • Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.