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Sino Biological™ SERPINA1 Recombinant Human Protein, His Tag DFS Item


This recombinant protein was expressed from a DNA sequence encoding the human SERPINA1 pre-protein (NP_000286.3) (Met 1-Lys 418) fused to the polyhistidine tag at the C-terminus

Manufacturer: life technologies  LS10306H08H5

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 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Catalog No. 10306H08H5

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Description & Specifications

Specifications

Format Lyophilized
Common Name Serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
Validated Application Assay Standard
Endotoxin Concentration <1 EU/μg
Protein Subtype Proteinase Inhibitor
Molecular Weight 45.7kDa
Storage Requirements Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Quantity 5μg
Gene Alias SerpinA1, PI, A1A, AAT, PI1, A1AT, mgC9222, PRO2275, mgC23330
Protein Family Protease Inhibitors
Protein Tag His Tag
Expression System Mammalian
Purity ≥90% by SDS-PAGE
Gene Symbol SERPINA1
For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories

Serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 (SERPINA1), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

  • Activity: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2
    ; the IC50 value is <3.0nM, as measured in 100μL reaction mixture containing 1.25ng trypsin, 10μM substrate, 50mM Tris, 10mM CaCl2, 0.15 M NaCl, pH 7.5
  • Formulation: Lyophilized in 25mM Tris, 150mM NaCl, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.