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Sino Biological™ SERPINA1 Recombinant Human Protein, His Tag DFS Item


This recombinant protein was expressed from a DNA sequence encoding the human SERPINA1 pre-protein (NP_000286.3) (Met 1-Lys 418) fused to the polyhistidine tag at the C-terminus

Manufacturer: life technologies  LS10306H08H250

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 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Catalog No. 10306H08HL

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Description & Specifications

Specifications

For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Format Lyophilized
Molecular Weight 45.7kDa
Purity ≥90% by SDS-PAGE
Quantity 5 x 50μg
Storage Requirements Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Protein Subtype Proteinase Inhibitor
Endotoxin Concentration <1 EU/μg
Gene Alias SerpinA1, PI, A1A, AAT, PI1, A1AT, mgC9222, PRO2275, mgC23330
Common Name Serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
Gene Symbol SERPINA1
Protein Tag His Tag
Validated Application Assay Standard
Protein Family Protease Inhibitors
Expression System Mammalian

Serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 (SERPINA1), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

  • Activity: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2
    ; the IC50 value is <3.0nM, as measured in 100μL reaction mixture containing 1.25ng trypsin, 10μM substrate, 50mM Tris, 10mM CaCl2, 0.15 M NaCl, pH 7.5
  • Formulation: Lyophilized in 25mM Tris, 150mM NaCl, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.