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Sino Biological™ SEMA4A Recombinant Human Protein, His Tag DFS Item


This recombinant protein was expressed from a DNA sequence encoding the extracellular domain of human SEMA4A (NP_071762.2) (Met 1-His 683) fused to a polyhistidine tag at the C-terminus

Manufacturer: life technologies  LS11756H08H5

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 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Catalog No. 11756H08H5

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Description & Specifications

Specifications

For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Format Lyophilized
Molecular Weight 73.4kDa
Purity ≥90% by SDS-PAGE
Quantity 5μg
Storage Requirements Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Protein Subtype Other Proteins
Endotoxin Concentration <1 EU/μg
Gene Alias RP11-54H19.2, CORD10, FLJ12287, RP35, SEMAB, SEMB
Common Name Sema domain, immunoglobulin domain (Ig), TM and short cytoplasmic domain, (semaphorin) 4A
Gene Symbol SEMA4A
Protein Tag His Tag
Validated Application Assay Standard
Protein Family Other Proteins
Expression System Mammalian

Sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4 (SEMA4A), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

  • Activity: Measured by its ability to bind biotinylated mouse SEMA4D in a functional ELISA
  • Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.