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Sino Biological™ S100A9 Recombinant Human Protein, His Tag DFS Item


This recombinant protein is expressed from a DNA sequence encoding the human S100A9 (NP_002956.1) (Met 1-Pro 114) fused to a polyhistidine tag at the C-terminus

Manufacturer: life technologies  LS11145H08B50

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 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Catalog No. 11145H08B50

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Description & Specifications

Specifications

For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Format Lyophilized
Molecular Weight 14.6kDa
Purity ≥90% by SDS-PAGE
Quantity 50μg
Storage Requirements Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Protein Subtype Other Proteins
Endotoxin Concentration <1 EU/μg
Gene Alias 60B8AG, CAGB, CFAG, CGLB, L1AG, LIAG, MAC387, MIF, MRP14, NIF, P14
Common Name S100 calcium binding protein A9
Gene Symbol S100A9
Protein Tag His Tag
Validated Application Assay Standard
Protein Family Other Proteins
Expression System Insect

S100 Calcium Binding Protein A9 (S100A9) recombinant human protein is supplied as a lyophilized powder. This protein is suitable for use in performing cell-based assays and protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.&line-break;Background: Human Protein S100-A9, also known as S100 calcium-binding protein A9, is a calcium-binding protein. It has anti-microbial activity towards bacteria and fungi. The anti-microbial and proapoptotic activity of S100A9 is inhibited by zinc ions. S100A9 plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS). It promotes tubulin polymerization when unphosphorylated. It also promotes phagocyte migration and infiltration of granulocytes at sites of wounding. S100A9 plays a role as a proinflammatory mediator in acute and chronic inflammation and upregulates the release of IL8 and cell-surface expression of ICAM1.

  • N-terminal Sequence Analysis: Met
  • Activity: 1. Measured by its ability to bind recombinant human S100A8-his in a functional ELISA; 2. Measured by its ability to bind recombinant human his-S100A8 in a functional ELISA; 3. Measured by its ability to inhibit the proliferation of MCF7 human breast adenocarcinoma cells; the ED50 for this effect is typically 10-30μg/mL
  • Formulation: lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% mannitol, 5% trehalose, 0.02% Tween®-80
  • Reconstitution: Dissolve the protein in sterile double-distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

Manufactured by Sino Biological (China).