Sino Biological™ S100A9 Recombinant Human Protein, His Tag
This recombinant protein is expressed from a DNA sequence encoding the human S100A9 (NP_002956.1) (Met 1-Pro 114) fused to a polyhistidine tag at the C-terminus
Manufacturer: Life Technologies LS11145H08B50
Protein; Sino Biological; S100A9 Recombinant Human Protein, His Tag; 50ug; Source: Human; Purity: greater than or equal to 90% by SDS-PAGES100 Calcium Binding Protein A9 (S100A9) recombinant human protein is supplied as a lyophilized powder. This protein is suitable for use in performing cell-based assays and protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.&line-break;Background: Human Protein S100-A9, also known as S100 calcium-binding protein A9, is a calcium-binding protein. It has anti-microbial activity towards bacteria and fungi. The anti-microbial and proapoptotic activity of S100A9 is inhibited by zinc ions. S100A9 plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS). It promotes tubulin polymerization when unphosphorylated. It also promotes phagocyte migration and infiltration of granulocytes at sites of wounding. S100A9 plays a role as a proinflammatory mediator in acute and chronic inflammation and upregulates the release of IL8 and cell-surface expression of ICAM1.
- N-terminal Sequence Analysis: Met
- Activity: 1. Measured by its ability to bind recombinant human S100A8-his in a functional ELISA; 2. Measured by its ability to bind recombinant human his-S100A8 in a functional ELISA; 3. Measured by its ability to inhibit the proliferation of MCF7 human breast adenocarcinoma cells; the ED50 for this effect is typically 10-30μg/mL
- Formulation: lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% mannitol, 5% trehalose, 0.02% Tween®-80
- Reconstitution: Dissolve the protein in sterile double-distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible
Manufactured by Sino Biological (China).
|Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories|
|60B8AG, CAGB, CFAG, CGLB, L1AG, LIAG, MAC387, MIF, MRP14, NIF, P14|
|≥90% by SDS-PAGE|
|Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.|
|S100 calcium binding protein A9|