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Sino Biological™ RSPO1 Recombinant Human Protein, His Tag DFS Item


This recombinant protein was expressed from a DNA sequence encoding the human RSPO1 (NP_001033722.1) (Met 1-Ala 263) fused to a polyhistidine tag at the C-terminus

Manufacturer: life technologies  LS11083H08H50

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 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Catalog No. 11083H08H50

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Description & Specifications

Specifications

For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Format Lyophilized
Molecular Weight 28.2kDa
Purity ≥90% by SDS-PAGE
Quantity 50μg
Storage Requirements Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Protein Subtype Wnt Proteins & Receptors|Other Proteins
Endotoxin Concentration <1 EU/μg
Gene Alias RP11-566C13.1, CRISTIN3, FLJ40906, RSPO
Common Name R-spondin 1
Gene Symbol RSPO1
Protein Tag His Tag
Validated Application Assay Standard
Protein Family Other Proteins
Expression System Mammalian

R-Spondin 1 (RSPO1), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

  • Activity: Measured by its binding ability in a functional ELISA: 1. Immobilized human RSPO1 at 20μg/mL (100μL/well) can bind human LIMPII with a linear range of 32-800ng/mL; 2. Immobilized human RSPO1 at 20μg/mL (100μL/well) can bind mouse CD36 with a linear range of 6.4-800ng/mL
  • Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.