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Sino Biological™ PDCD1 Recombinant Human Protein, His Tag DFS Item


This recombinant protein is expressed from a DNA sequence encoding the extracellular domain (Met 1-Gln 167) of human PDCD1 (NP_005009.2) fused to a polyhistidine tag at the C-terminus

Manufacturer: life technologies  LS10377H08H5

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 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Catalog No. 10377H08H5

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Description & Specifications

Specifications

For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Format Lyophilized
Molecular Weight 18kDa
Purity ≥85% by SDS-PAGE
Quantity 5μg
Storage Requirements Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Protein Subtype Other Proteins
Endotoxin Concentration <1 EU/μg
Gene Alias PDCD1, PD1, CD279, SLEB2, hPD-1, hPD-l
Common Name Programmed cell death 1
Gene Symbol PDCD1
Protein Tag His Tag
Validated Application Assay Standard
Protein Family CD Proteins
Expression System Mammalian

Programmed cell death 1 (PDCD1) recombinant human protein is supplied as a lyophilized powder. This protein is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.&line-break;Background: Programmed Cell Death 1 is a type I transmembrane immunoreceptor and belongs to the CD28 immunoglobulin superfamily that mediates immune responses. PD1 is expressed on activated T-cells, B-cells, myeloid cells and a subset of thymocytes. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. PD1 has been suggested to be involved in lymphocyte clonal selection and peripheral tolerance, and thus contributes to the prevention of autoimmune diseases. Furthermore, PD1 is shown to be a regulator of virus-specific CD8+ T cell survival in HIV infection.

  • N-terminal Sequence Analysis: Leu25
  • Activity: measured by its binding ability in a functional ELISA; immobilized human PDCD1 at 10μg/mL (100μL/well) has been shown to bind recombinant human CD274-Fc chimera with a linear range of 0.02-0.4μg/mL
  • Formulation: lyophilized in 20mM Tris, 500mM NaCl, 500mM imidazole, pH 7.4, 5% mannitol, 5% trehalose, 0.02% Tween®-80
  • Reconstitution: Dissolve the protein in sterile double-distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

Manufactured by Sino Biological (China).