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Sino Biological™ PCSK9 Recombinant Human Protein, His Tag DFS Item


This recombinant protein was expressed from a DNA sequence encoding the human PCSK9 (NP_777596.2) (Met 1-Gln 692) fused to a polyhistidine tag at the C-terminus

Manufacturer: life technologies  LS10594H08H5

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 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Catalog No. 10594H08H5

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Description & Specifications

Specifications

For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Format Lyophilized
Molecular Weight 20kDa
Purity ≥90% by SDS-PAGE
Quantity 5μg
Storage Requirements Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Protein Subtype Proteases
Endotoxin Concentration <1 EU/μg
Gene Alias PCSK9, FH3, NARC1, LDLCQ1, NARC-1, HCHOLA3
Common Name Proprotein convertase subtilisin/kexin type 9
Gene Symbol PCSK9
Protein Tag His Tag
Validated Application Assay Standard
Protein Family Other Proteins
Expression System Mammalian

Proprotein convertase subtilisin/kexin type 9 (PCSK9), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

  • Activity: Measure by its ability to bind with human LDLR in a functional ELISA; immobilized recombinant human PCSK9 at 10μg/mL (100μL/well) can bind biotinylated human LDLR, the EC50 of biotinylated human LDLR is 0.61μg/mL
  • Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.