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Sino Biological™ PARP1 Recombinant Human Protein, His Tag DFS Item


This recombinant protein was expressed from the amino acids corresponding to the full length of human PARP1 (NP_001609.2) (Met 1-Trp 1014) fused to a polyhistidine tag at the C-terminus

Manufacturer: life technologies  LS11040H08B5

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 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Catalog No. 11040H08B5

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Description & Specifications

Specifications

For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Format Frozen
Molecular Weight 114.5kDa
Purity ≥90% by SDS-PAGE
Quantity 5μg
Storage Requirements Store at -80°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Protein Subtype Other Proteins
Endotoxin Concentration <1 EU/μg
Gene Alias PARP1, RP11-125A15.2, ADPRT, ADPRT1, PARP, PARP-1, PPOL, pADPRT-1
Common Name Poly (ADP-ribose) polymerase 1
Gene Symbol PARP1
Protein Tag His Tag
Validated Application Assay Standard
Expression System Insect

Poly (Adp-Ribose) Polymerase 1 (PARP1), recombinant human protein is supplied in frozen format. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

  • Activity: Measured by its binding ability in a functional ELISA; immobilized human PARP1 at 10μg/mL (100μL/well) can bind to biotinylated human HSP70, the EC50 of biotinylated human HSP70 is 0.035μg/mL
  • Formulation: 50mM Tris, 100mM NaCl, pH 8, 2mM EDTA, 2mM PMSF, 0.5mM TCEP, 10% glycerol
  • Reconstitution: No reconstitution needed

This protein is manufactured by Sino Biological Inc.