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Sino Biological™ MET (c-MET) Recombinant Human Protein (without Catalytic Activity Domain), His Tag DFS Item


This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Thr 932) of human MET (NP_000236) fused to a polyhistidine tag at the C-terminus

Manufacturer: life technologies  LS10692H08H250

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 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Catalog No. 10692H08HL

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Description & Specifications

Specifications

For Use With (Application) Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
Format Lyophilized
Gene ID (Entrez) 4233
Molecular Weight 103.0kDa
Purity ≥85% by SDS-PAGE
Quantity 5 x 50μg
Storage Requirements Store at -20° to -70°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed.
Structural Form Truncated/Recombinant
Protein Subtype Receptor Tyrosine Kinases|Growth Factor Receptors
Endotoxin Concentration <1 EU/μg
Gene Alias MET, AUTS9, HGFR, RCCP2, c-Met
Common Name Met proto-oncogene
Gene Symbol MET
Protein Tag His Tag
Validated Application Assay Standard
Protein Family Growth Factors & Receptors
Expression System Mammalian
KinaseFamily MET (HGFR) Kinase Family

Met proto-oncogene/hepatocyte growth factor receptor (MET/c-MET), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications. Since this product is a truncated protein and does not contain intracellular catalytic activity domain, it is not suitable for use in enzyme activity studies.

  • Activity: Measured by its binding ability in a functional ELISA; immobilized recombinant human HGF at 10μg/mL (100μL/well) can bind biotinylated MET, the ED50 of biotinylated Met is 5.28μg/mL
  • Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween-80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

Shipping Condition: Room Temperature. This protein is manufactured by Sino Biological Inc.