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Sino Biological™ MCAM Recombinant Human Protein, His Tag DFS Item

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain of human MCAM precursor (NP_006491.2) (Met 1-Gly 559) fused to a C-terminal polyhistidine tag

Manufacturer:  Life Technologies LS10115H08H25

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Catalog No. 10115H08H25


 

Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Specifications

Description

Protein; Sino Biological; MCAM Recombinant Human Protein, His Tag; 5 x 5ug; Source: Human; Purity: greater than or equal to 90% by SDS-PAGE

Melanoma Cell Adhesion Molecule (MCAM), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.
  • Activity: Measured by the ability of the immobilized protein to support the adhesion of the HUVEC human umbilical vein endothelial cell line; when 3 x 104 cells/well are added to human MCAM coated plates (0.8μg/mL, 100μL/well), approximately >50% will adhere after one hour at 37°C
  • Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
  • Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

This protein is manufactured by Sino Biological Inc.

Description & Specifications

Specifications

Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories
61.3kDa
5 x 5μg
Intercellular Adhesion Molecule
MCAM, CD146, MUC18
MCAM
CD Proteins
His Tag
Lyophilized
≥90% by SDS-PAGE
Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.Expires one year from date of receipt when stored as instructed.
<1 EU/μg
Melanoma Cell Adhesion Molecule
Assay Standard
Mammalian